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Liquid Chromatography/Ultra Violet/Mass Spectrometric and Liquid Chromatography/Nuclear Magnetic Resonance Spectroscopic Analysis of Crude Extracts of Gentianaceae Species
Author(s) -
Wolfender JeanLuc,
Rodriguez Sylvain,
Hostettmann Kurt,
Hiller Wolf
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199705)8:3<97::aid-pca350>3.0.co;2-z
Subject(s) - chemistry , chromatography , gentianaceae , high performance liquid chromatography , mass spectrometry , gentiana , nuclear magnetic resonance spectroscopy , liquid chromatography–mass spectrometry , proton nmr , organic chemistry , botany , biology
High performance liquid chromatography (HPLC) coupled with ultra violet photodiode array (LC/UV‐DAD) and mass spectrometry (LC/MS) detection has been used routinely for the chemical screening of crude extracts of Gentianaceae species. The recent progress realised in the field of nuclear magnetic resonance spectroscopy (NMR) has given a new impulse to the coupling of this detection method with liquid chromatography (LC/NMR) and several LC/NMR probes are now commercially available. In order to evaluate the potential of this coupled technique as a complement to LC/UV/MS for the on‐line identification of plant metabolites, two characteristic Gentianaceae species were analysed, namely Swertia calycina and Gentiana ottonis . On‐line UV, MS, MS/MS 1 H‐NMR and 1 H‐ 1 H‐NMR spectra of the major constituents of these two plants allowed their identification directly within the crude extracts. The potential and the limitations of LC/NMR used together with LC/UV/MS for the on‐line analysis of plant metabolites are discussed. © 1997 John Wiley & Sons, Ltd.