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Quantitation of the Anti‐HIV Alkaloids Michellamines A and B by High Pressure Liquid Chromatography
Author(s) -
McCloud Thomas G.,
Britt John R.,
Cartner Laura K.,
Pearl Kay C.,
Majadly Fathy D.,
Muschik Gary M.,
Klueh Paul A.,
Cragg Gordon M.,
Thomas Duncan W.,
Jato Johnson G.,
Simon James E.
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199705)8:3<120::aid-pca345>3.0.co;2-4
Subject(s) - chemistry , human immunodeficiency virus (hiv) , chromatography , high performance liquid chromatography , in vitro , genus , alkaloid , stereochemistry , botany , biochemistry , virology , biology
Michellamines A and B, naphthylisoquinoline dimeric alkaloids from the leaf of a previously undescribed species of the genus Ancistrocladus , show anti‐human immunodeficiency virus (HIV) activity in an in vitro screen. In order to determine the distribution of these alkaloids in the genus, the level of production in the new species, and to gain an understanding of the horticultural aspects of production, a sensitive high pressure liquid chromatography analytical method requiring a small biomass was developed. This method resolved michellamines A and B and congeners and enabled quantitation of each. The production of the michellamines was found to be limited to the new species, A. korupensis . © 1997 John Wiley & Sons, Ltd.