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Quantitative Analysis of Norditerpenoid Alkaloids in Larkspur ( Delphinium spp.) by Fourier Transform Infrared Spectroscopy
Author(s) -
Gardner Dale R.,
Manners Gary D.,
Ralphs Michael H.,
Pfister James A.
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199703)8:2<55::aid-pca338>3.0.co;2-q
Subject(s) - delphinium , chemistry , alkaloid , fourier transform infrared spectroscopy , chromatography , ranunculaceae , botany , infrared spectroscopy , stereochemistry , organic chemistry , biology , quantum mechanics , physics
A Fourier transform infrared (FT–IR) spectroscopic method for measuring norditerpenoid alkaloids in larkspurs was developed. Alkaloids were isolated from dry plant material using a simplified extraction procedure and quantitated using FT–IR. Calibration of the method was made from selected larkspur samples with known alkaloid levels determined by high pressure liquid chromatography and gravimetric methods. Thirty‐nine samples from three different larkspur species ( Delphinium barbeyi, D. glaucescens, and D. occidentale ) were analysed to demonstrate the ability of the FT–IR method to measure toxic and total alkaloid concentrations in these plants. © 1997 by John Wiley & Sons, Ltd.