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High Performance Liquid Chromatography and Photodiode Array Detection of Phenylpropanoids and Benzoates in Rubus Protoplasts Elicited by Kinetin
Author(s) -
Chevolot Lionel,
DuboisDauphin Robin,
Yvin Jean Claude,
Liénart Yvette
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199701)8:1<22::aid-pca329>3.0.co;2-2
Subject(s) - chemistry , chromatography , high performance liquid chromatography , kinetin , elution , methanol , biochemistry , organic chemistry , explant culture , in vitro
Reverse‐phase high performance liquid chromatography with photodiode array detection (PAD) has been developed for simultaneous separation, identification and quantification of picomoles of phenylalanine ammonia‐lyase (PAL) reaction products. Separation has been achieved in 30 min on a reverse‐phase silica column using methanol:water gradient elution, and PAD provided sensitive and specific determination of phenolics. The method has been applied to biological samples to study kinetin‐induced changes in phenol pathways within protoplasts of Rubus fruticosus (raspberry). © 1997 by John Wiley & Sons, Ltd.