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Fatty Acid Anilides as Internal Standards for High Performance Liquid Chromatographic Analyses of Valeriana officinalis L. and Other Medicinal Plants
Author(s) -
Perry Nigel B.,
Burgess Elaine J.,
Lorimer Stephen D.,
van Klink John W.
Publication year - 1996
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199609)7:5<263::aid-pca311>3.0.co;2-s
Subject(s) - chemistry , high performance liquid chromatography , chromatography , acetonitrile , fatty acid , officinalis , extraction (chemistry) , organic chemistry , botany , biology
Fatty acid anilides are proposed as internal standards for high performance liquid chromatographic (HPLC) analyses of medicinal plants. A one‐pot synthesis of the C 5 to C 12 anilides is described. Reverse‐phase HPLC showed that the synthesized anilides covered the optimum range of k ′ values for mobile phases ranging from 50 to 90% acetonitrile in water. There was an approximately linear relationship between fatty acid chain length and log k ′ under a variety of isocratic conditions. For a linear gradient of mobile phase composition, there was an approximately linear relationship between fatty acid chain length and k ′. The development of an extraction and HPLC analysis method for the biologically active components valtrate and valerenic acid in Valeriana officinalis is described including use of C 11 fatty acid anilide as an internal standard. The levels of these components were similar in both fresh and dried valerian.