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The Development and Evaluation of Capillary Electrophoretic Methods for the Determination of the Major Phenolic Constituents of Potato ( Solanum tuberosum ) Tubers
Author(s) -
Fernandes Joao B.,
Griffiths D. Wynne,
Bain Henry,
Fernandes Fabiano A. N.
Publication year - 1996
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199609)7:5<253::aid-pca307>3.0.co;2-c
Subject(s) - chemistry , solanum tuberosum , capillary electrophoresis , chromatography , electrophoresis , botany , biology
A range of capillary electrophoretic methods utilizing both capillary zone (CZE) and micellar electrokinetic techniques were developed for the separation of 15 compounds consisting of a complex micture of coumarins, cinnamic and phenolic acids. Factors influencing their migration times are evaluated and discussed, and conditions optimized for their quantitative analyses. These techniques were applied to potato extracts, in which chlorogenic acid and caffeic acids were identified and simultaneously quantified. A CZE method was also developed for the separation of chlorogenic acid isomers. These techniques were utilized in a preliminary study to evaluate the effect of light on both the isomeric ratios and total chlorogenic acid content of potato tubers. The results indicated that prolonged exposure to light resulted not only in an increase in total chlorogenic acid content but also in a significant change in the relative proportions of the two major chlorogenic isomers present in the tubers.