Premium
Separation and Identification of Plant Glycerolipid Molecular Species by Particle Beam High Performance Liquid Chromatography–Mass Spectrometry
Author(s) -
Nilsson Ralf,
Liljenberg Conny
Publication year - 1996
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199609)7:5<228::aid-pca314>3.0.co;2-8
Subject(s) - chemistry , mass spectrometry , chromatography , phosphatidylethanolamine , analytical chemistry (journal) , quadrupole mass analyzer , phosphatidylcholine , high performance liquid chromatography , chemical ionization , mass spectrum , particle (ecology) , ionization , ion , phospholipid , organic chemistry , membrane , biochemistry , oceanography , geology
A high performance liquid chromatograph was connected to a quadrupole mass spectrometer through a particle beam interface in order to investigate the suitability of the interface for the analysis of plant glycerolipid molecular species. Separation was performed on a reversed phase column with a high carbon loading (20%), together with mobile phase systems containing little or no water for optimal interface performance. Positive chemical ionization mass spectra which allowed unambiguous determination of molecular species were obtained for triacylglycerols, diacylglycerols, digalactosyldiacylglycerols, phosphatidylcholine and phosphatidylethanolamine. Baseline separation for all species was achieved in the case of triacylglycerols and diacylglycerols. Polar lipids could not be baseline separated when the mass spectrometer was used as the detector due to a peak broadening effect in the ion source.