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Resolution of Racemic Jasmonic Acid and Abscisic Acid by Chiral High Performance Liquid Chromatography
Author(s) -
Kramell Robert,
Schneider Gernot,
Miersch Otto
Publication year - 1996
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199607)7:4<209::aid-pca292>3.0.co;2-h
Subject(s) - chemistry , enantiomer , abscisic acid , jasmonic acid , resolution (logic) , chromatography , circular dichroism , methyl jasmonate , jasmonate , chiral resolution , high performance liquid chromatography , derivative (finance) , organic chemistry , stereochemistry , biochemistry , artificial intelligence , computer science , gene , arabidopsis , mutant , financial economics , economics
The direct resolution of racemic jasmonic acid (JA) and abscisic acid (ABA), as the free acids, into the corresponding enantiomers by high performance liquid chromatography on a Nucleodex β‐PM column is reported. The resolution factors ( R s JA =1.38; R s ABA =1.65) were such that the method could be applied to the separation of these racemates on a preparative scale. The racemates of methyl jasmonate and its 12‐hydroxy‐derivative could be resolved as their respective methyl esters. The pure isomers obtained were characterized using circular dichroism and optical rotatory dispersion.