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Sample Preparation of Standardized Extracts of Ginkgo biloba by Supercritical Fluid Extraction
Author(s) -
van Beek Teris A.,
Taylor Larry T.
Publication year - 1996
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199607)7:4<185::aid-pca302>3.0.co;2-u
Subject(s) - chemistry , chromatography , supercritical fluid extraction , sample preparation , ginkgolides , extraction (chemistry) , silica gel , solid phase extraction , cartridge , supercritical fluid chromatography , methanol , gas chromatography , ginkgo biloba , organic chemistry , medicine , mechanical engineering , pharmacology , engineering
A method of sample preparation of standardized extracts of Ginkgo biloba using supercritical fluid extraction (SFE) is described. Ginkgolides and bilobalide could be selectively extracted with carbon dioxide containing 10% methanol at 335 atm and 45 °C from a methanolic solution of the extract. An in‐line filter of silica gel was found to be essential for obtaining clean samples. Trapping was carried out with a solid silica gel trap at 80 °C. After eluting the trap with methyl acetate, the sample could be analysed by gas liquid chromatography or high performance liquid chromatography. Recoveries of the five terpenes relative to a standard solid phase extraction (SPE) procedure varied for two different extracts from 98.6 to 102.3%. Relative standard deviations were better for SFE than for SPE. A further advantage of the SFE over the SPE method is that it is much less laborious. A disadvantage is that it requires an automated supercritical extractor. With a small adaptation, the SFE method could also be used for finished ginkgo drugs in an aqueous alcoholic solution.