Premium
The evaluation of capillary zone and micellar electrokinetic capillary chromatographic techniques for the simultaneous determination of flavonoids, cinnamic and phenolic acids in blackcurrant ( Ribes nigrum ) bud extracts
Author(s) -
Fernandes Joao B.,
Griffith D. Wynne,
Bain Henry
Publication year - 1996
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199603)7:2<97::aid-pca286>3.0.co;2-m
Subject(s) - chemistry , ribes , chromatography , capillary electrophoresis , micellar electrokinetic chromatography , cinnamic acid , capillary action , electrokinetic phenomena , organic chemistry , botany , materials science , composite material , biology
A range of capillary electrophoretic methods utilizing both capillary zone and micellar electrokinetic techniques were evaluated for the simultaneous separation of 15 compbunds consisting of a complex mixture of flavonoids, cinnamic acids and simple phenolic acids. Factors influencing their migration times are evaluated and discussed, and conditions optimised for their quantitative analysis. These techniques were applied to crude methanolic blackcurrant bud extracts in which 11 compounds were directly identified and simultaneously quantified.