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Analysis of Constituents of Iris Rhizomes. Part III—Packed Column Supercritical Fluid Chromatography and High Pressure Liquid Chromatography of Iridals from Rhizomes of Iris pallida L.
Author(s) -
Bicchi Carlo,
Rubiolo Patrizia,
Fresia Monica,
David Frank,
Sandra Pat
Publication year - 1996
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199601)7:1<37::aid-pca281>3.0.co;2-9
Subject(s) - chemistry , chromatography , supercritical fluid chromatography , rhizome , high performance liquid chromatography , countercurrent chromatography , column chromatography , chromatography column , supercritical fluid , silica gel , chiral column chromatography , organic chemistry , ecology , biology
Iridals constitute a class of triterpenoids characteristic of the genus Iris which have been demonstrated to be the precursors of irones, a group of compounds widely and successfully used in the perfume industry because of their attractive violet smell. Packed column supercritical fluid chromatography with ultra violet detection was applied to the analysis of the iridal fraction isolated from rhizomes of Iris pallida L. Various chromatographic conditions were applied (different columns, column coupling and stationary phase coupling, different density programmes, and several modifiers and modifier programmes) in order to optimize iridal separation. C18‐ and CN‐stationary phases were successful in iridal separation. A comparison in terms of resolution and analysis time with the results obtained from high pressure liquid chromatography is reported.