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31 P and 1 H NMR spectroscopic studies of liver extracts of carbon tetrachloride‐treated rats
Author(s) -
Harvey Peta J.,
Gready Jill E.,
Hickey Haruyo M.,
Le Couteur David G.,
McLean Allan J.
Publication year - 1999
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199910)12:6<395::aid-nbm568>3.0.co;2-m
Subject(s) - carbon tetrachloride , medicine , cirrhosis , endocrinology , chemistry , metabolism , phosphomonoesters , hepatocyte , hypoxia (environmental) , phosphate , biochemistry , biology , inorganic phosphate , oxygen , organic chemistry , in vitro
NMR spectroscopy was used to examine hepatic metabolism in cirrhosis with a particular focus on markers of functional cellular hypoxia. 31 P and 1 H NMR spectra were obtained from liver extracts from control rats and from rats with carbon tetrachloride‐induced cirrhosis. A decrease of 34% in total phosphorus content was observed in cirrhotic rats, parallelling a reduction of 40% in hepatocyte mass as determined by morphometric analysis. Hypoxia appeared to be present in cirrhotic rats, as evidenced by increased inorganic phosphate levels, decreased ATP levels, decreased ATP:ADP ratios (1.72 ± 0.40 vs 2.48 ± 0.50, p < 0.01), and increased inorganic phosphate:ATP ratios (2.77 ± 0.48 vs 1.62 ± 0.24, p < 0.00001). When expressed as a percentage of the total phosphorus content, higher levels of phosphoethanolamine and lower levels of NAD and glycerophosphoethanolamine were detected in cirrhotic rats. Cirrhotic rats also had increased phosphomonoester:phosphodiester ratios (5.73 ± 2.88 vs 2.53 ± 0.52, p < 0.01). These findings are indicative of extensive changes in cellular metabolism in the cirrhotic liver, with many findings attributable to the presence of intracellular hypoxia. Copyright © 1999 John Wiley & Sons, Ltd.