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Using the β/α peak‐height ratio of ATP in 31 P NMR spectra to measure free [Mg 2+ ]: theoretical and practical problems
Author(s) -
Mulquiney Peter J.,
Kuchel Philip W.
Publication year - 1999
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199906)12:4<217::aid-nbm564>3.0.co;2-5
Subject(s) - measure (data warehouse) , nuclear magnetic resonance spectroscopy , beta (programming language) , alpha (finance) , sensitivity (control systems) , chemistry , calibration , in vivo , analytical chemistry (journal) , nuclear magnetic resonance , physics , mathematics , chromatography , statistics , computer science , biology , data mining , construct validity , programming language , psychometrics , microbiology and biotechnology , electronic engineering , engineering
A recently described alternative method for determining free [Mg 2+ ] in cells and tissue with 31 P NMR spectroscopy, which is based on the peak‐height ratio of the β and β resonances of ATP ( h β/α ) [ J. Biol. Chem . 271 , 21141–21150 (1996)], was found to have numerous problems. These problems include a flaw in the basic multiple equilibrium analysis, the inapplicability of the calibration curve to in vivo studies, and a lack of sensitivity of the measured ratio to changes in free [Mg 2+ ] at ‘clinical’ field strengths. This suggests that the use of this procedure will be very limited. Copyright © 1999 John Wiley & Sons, Ltd.