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Esophageal cancer phospholipids correlated with histopathologic findings: a 31 P NMR study
Author(s) -
Merchant Thomas E.,
Minsky Bruce D.,
Lauwers Gregory Y.,
Diamantis Pamela M.,
Haida Toni,
Glonek Thomas
Publication year - 1999
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199906)12:4<184::aid-nbm560>3.0.co;2-m
Subject(s) - phospholipid , plasmalogen , lysophosphatidylcholine , lysophosphatidic acid , chemistry , nuclear magnetic resonance , esophageal cancer , nuclear magnetic resonance spectroscopy , phosphatidylcholine , pathology , cancer , biochemistry , medicine , membrane , organic chemistry , physics , receptor
We analyzed 36 esophageal tumor specimens for phospholipid content using phosphorus nuclear magnetic resonance spectroscopy ( 31 P NMR) and correlated the individual phospholipid profiles with specific clinical and histopathologic features. Among the 18 phospholipids identified in the esophageal tumor specimens, the mean mole percentage concentration of dimethylphosphatidylethanolamine, lysoalkylacylphosphatidylcholine, lysophosphatidic acid, lysophosphatidylcholine (deacylated at the glycerol‐1 carbon), and lysoethanolamine plasmalogen correlated with pathologic T stage, nuclear grade, or the presence of lymphatic invasion. 31 P NMR produces well‐dispersed phospholipid spectra and a precise determination of phospholipid relative mole percentages. These data provide a statistical correlation between histopathologic features and molecules known to play an important role in cellular activities and processes unique to malignant tissues. Copyright © 1999 John Wiley & Sons, Ltd.