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Spatial distribution of deoxy myoglobin in human muscle: an index of local tissue oxygenation
Author(s) -
Tran TuanKhanh,
Sailasuta Napapon,
Hurd Ralph,
Jue Thomas
Publication year - 1999
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199902)12:1<26::aid-nbm563>3.0.co;2-9
Subject(s) - myoglobin , nuclear magnetic resonance , skeletal muscle , chemistry , oxygenation , magnetic resonance imaging , biophysics , anatomy , biochemistry , physics , medicine , biology , radiology
The proximal histidyl N δ H signal of myoglobin is detectable in 1 H NMR spectra of myocardial and skeletal muscle, and its intensity reflects the intracellular oxygenation. At 1.5 Tesla (T), the typical field strength of clinical magnetic resonance imaging (MRI) magnets, the paramagnetic relaxation contribution decreases sufficiently to permit the implementation of chemical shift imaging technique to map the spatial distribution of the deoxy Mb N δ H signal from human gastrocnemius muscle. One and two‐dimensional chemical shift imaging experiments reveal clearly the localized deoxy Mb signal in muscle and consequently the spatial distribution of the cellular oxygenation. The results indicate the feasibility to assess the pO 2 in tissue regions and to directly study the regulation of oxidative metabolism in human tissue. Copyright © 1999 John Wiley & Sons, Ltd.