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Two‐compartment model for determination of glycolytic rates of solid tumors by in vivo 13 C NMR spectroscopy
Author(s) -
Artemov Dmitri,
Bhujwalla Zaver M.,
Pilatus Ulrich,
Glickson Jerry D.
Publication year - 1998
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199812)11:8<395::aid-nbm536>3.0.co;2-r
Subject(s) - glycolysis , nuclear magnetic resonance spectroscopy , compartment (ship) , chemistry , intracellular , in vivo , limiting , anaerobic glycolysis , biophysics , metabolism , biochemistry , biology , stereochemistry , mechanical engineering , oceanography , microbiology and biotechnology , engineering , geology
Carbon‐13 NMR spectroscopy of 13 C enriched substrates is useful for non‐invasively determining metabolic fluxes of cells and tissues. Our study demonstrates that for RIF‐1 tumor cells, examined under monolayer culture with continuous perfusion and also grown as solid subcutaneously (sc) implanted tumors in vivo , the levels of intracellular glucose and intermediates of the glycolytic pathway are below the level of detection by NMR spectroscopy. For these tumors, glucose transport into the cell is the most probable rate limiting step of the glycolytic pathway. Under these limiting conditions a simple two‐compartment model of glycolysis applies. This model yields two parameters: the average rate of glycolysis and the rate of lactate clearance through the vasculature. For the RIF‐1 tumor these parameters were 0.022 ± 0.01 and 0.034 ± 0.006 min −1 , respectively. Copyright © 1998 John Wiley & Sons, Ltd.