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Nuclear magnetic resonance studies of cesium‐133 in the halophilic halotolerant bacterium Ba 1 . Chemical shift and transport studies
Author(s) -
Sakhnini As'ad,
Gilboa Haggai
Publication year - 1998
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199804)11:2<80::aid-nbm505>3.0.co;2-i
Subject(s) - caesium , chemistry , intracellular , extracellular , potassium , sodium , ectoine , reagent , nuclear chemistry , radiochemistry , biophysics , inorganic chemistry , biochemistry , biology , osmoprotectant , organic chemistry , amino acid , proline
Ba 1 bacteria ( Holomonas israelensis ) were grown on different salt concentrations 0.2–4 M . When the cells were transferred to a medium containing 25 m M CsCl without potassium there was an uptake of cesium by the cells. The intracellular cesium signal was shifted from the cesium signal in the medium without the use of a shift reagent. The shift was depended on the salt concentration in the growth medium. The intracellular cesium shift showed a much smaller dependence on the concentration of salts in the medium than the extracellular cesium; the same results were detected for cells grown on a medium containing 25 m M CsCl. The cesium transport through the cell membrane was mostly by active transport. The cesium concentration in the cell was higher than that of the medium, ∼100 m M intracellular concentration compared to 25 m M in the medium. The first order constants for influx or efflux of cesium were from 2 × 10 −4 and up to 24 × 10 −4 /min for the different medium concentrations. © 1998 John Wiley & Sons, Ltd.