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Nuclear magnetic resonance studies of boar seminal plasma. Problems encountered in the identification of small molecules: hypotaurine and carnitine
Author(s) -
Kalic Marianne,
Kamp Günter,
Lauterwein Jürgen
Publication year - 1997
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199710)10:7<341::aid-nbm464>3.0.co;2-m
Subject(s) - hypotaurine , identification (biology) , boar , nuclear magnetic resonance , chemistry , taurine , physics , biology , semen , biochemistry , anatomy , botany , amino acid
Two major components in boar seminal plasma were assigned by 1 H and 13 C nuclear magnetic resonance spectroscopy. The first, previously called substance X (see Ref. 1, Biochim. Biophys. Acta 1243, 101–109 (1995)), was identified with difficulty as hypotaurine. This pointed to general difficulties in the NMR assignments of small molecules in mixtures of substances, even at the highest magnetic fields. In contrast, the identification of the second component as carnitine was obtained in a straightforward manner by total correlation spectroscopy and proton‐detected 13 C chemical shift correlation methods (gradient‐selected heteronuclear single quantum coherence and heteronuclear multiple bond correlation). Carnitine is known as a transporter of fatty acids through membranes. Both compounds were ultimately confirmed by addition of the authentic compounds. © 1997 John Wiley & Sons, Ltd.