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Imaging H 2 17 O distribution in a phantom and measurement of metabolically produced H 2 17 O in live mice by proton NMR
Author(s) -
Ronen Itamar,
Lee JingHuei,
Merkle Hellmut,
Ugurbil Kamil,
Navon Gil
Publication year - 1997
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199710)10:7<333::aid-nbm465>3.0.co;2-e
Subject(s) - imaging phantom , proton , chemistry , materials science , physics , nuclear physics , optics
Previously we suggested a method for indirect detection of H 2 17 O via a combination of 1 H spin–echo sequence and 17 O decoupling. The method is based on the shortening of the water proton T 2 due to the residual spin–spin coupling with the 17 O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the 17 O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H 2 17 O, and report for the first time an application of this method to in vivo detection of H 2 17 O in live newborn mice, where the H 2 17 O was introduced either directly i.p. or by inhalation of 17 O 2 where it was formed by oxidative metabolic activity. © 1997 John Wiley & Sons, Ltd.