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Optical isometric force transducer for measurement of rat skeletal muscle contraction in the NMR spectrometer
Author(s) -
Yamane Kazusuke,
Mitsumori Fumiyuki
Publication year - 1997
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199709)10:6<292::aid-nbm486>3.0.co;2-6
Subject(s) - spectrometer , isometric exercise , transducer , phosphocreatine , force transducer , chemistry , gastrocnemius muscle , muscle contraction , contraction (grammar) , skeletal muscle , nuclear magnetic resonance , materials science , analytical chemistry (journal) , anatomy , physics , optics , chromatography , acoustics , biology , physiology , endocrinology , energy metabolism
In order to measure force of contraction of the rat gastrocnemius muscle in the NMR spectrometer with a simultaneous observation of 31 P NMR spectra, an optical force transducer was constructed using gratings and optical fibers. Muscle contraction was accurately recorded in the spectrometer without interfering with NMR measurements. The half height width of the phosphocreatine (PCr) peak in the presence or absence of the transducer was 5 Hz. Decrease in PCr and increase in inorganic phosphate (Pi) in the muscle were successfully followed during monitoring of the force developed during exercise. The change in the PCr:(PCr+Pi) ratio correlated well with the time–force integral from the optical force transducer. In general, there was no interference with the 31 P NMR spectrum when the transducer was attached to rat leg, so this optical force transducer should prove valuable for studying muscle physiology in the NMR spectrometer. © 1997 John Wiley & Sons, Ltd.