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Diagnosis of a case of acute chloroquine poisoning using 1 H NMR spectroscopy: characterisation of drug metabolites in urine
Author(s) -
Maschke Susanna,
Azaroual Nathalie,
Wieruszeski JeanMichel,
Lippens Guy,
Imbenotte Michel,
Mathieu Daniel,
Vermeersch Gaston,
Lhermitte Michel
Publication year - 1997
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199709)10:6<277::aid-nbm479>3.0.co;2-m
Subject(s) - metabolite , urine , nuclear magnetic resonance spectroscopy , biological fluids , chemistry , drug , chloroquine , proton nmr , chromatography , pharmacology , nuclear magnetic resonance , medicine , stereochemistry , pathology , biochemistry , physics , malaria
Analysis of biological fluids by proton nuclear magnetic resonance spectroscopy ( 1 H NMR) is a promising tool in clinical biology. We have used this method for a rapid toxicological screening in the case of a suicide attempt. A urine sample was analysed at 300 and 600 MHz by 1D and 2D sequences (J‐resolved and TOCSY) in a short experimental time. Quantification was realized by peak integration of the 1D spectrum. The results showed the presence of chloroquine and its major metabolite monodesethylchloroquine at concentrations of 462 and 140 mg/L, respectively. Ethanol was also detected in the spectrum. It can be concluded that 1 H NMR provides many advantages as a tool for clinical diagnosis in a case of acute intoxication. © 1997 John Wiley & Sons, Ltd.