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Metabolic Changes Associated with Vacuolation in Murine Models of Scrapie using In Vitro 1 H‐NMR Spectroscopy
Author(s) -
Chung YuenLi,
Williams Alun,
Chong Angela,
Hope James,
Willims Steve C. R.,
Bell Jimmy D.
Publication year - 1996
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199612)9:8<359::aid-nbm428>3.0.co;2-d
Subject(s) - biology , creatine , nuclear magnetic resonance spectroscopy , gliosis , scrapie , choline , in vitro , phosphocholine , microbiology and biotechnology , chemistry , pathology , biochemistry , medicine , stereochemistry , prion protein , phospholipid , disease , neuroscience , membrane , phosphatidylcholine
In this study, metabolic changes in the 79A/C3H, ME7/VM, ME7/C3H, 87V/VM and 22A/SV scrapie mouse models were investigated during the clinical phase of the disease, using in vitro proton nuclear magnetic resonance spectroscopy. N ‐acetyl‐aspartate was found to be significantly reduced in infected mice of the ME7/C3H (40% reduction), ME7/VM (26%) and 79A/C3H (17%) models when compared to control mice, but not in the 87V/VM and 22A/SV models. The concentration of choline containing compounds and creatine remain unchanged in all models when compared with control murine brains. The level of N ‐acetyl‐aspartate reduction correlated with the extent of grey‐matter brain vacuolation. The levels of myo ‐inositol were found to be significantly increased in the ME7/VM (143%) and 79A/C3H (132%) models only and no significant changes were observed in the ME7/C3H, 87V/VM and 22A/SV models. These changes did not correspond to the severity of gliosis