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Effect of profound ischaemia on human muscle: MRI, phosphorus MRS and near‐infrared studies
Author(s) -
Hajnal Joseph V.,
Roberts Idris,
Wilson Jason,
Oatridge Angela,
Saeed Nadeem,
Cox I. Jane,
AlaKorpela Mika,
Bydder Graeme M.,
Young Ian R.
Publication year - 1996
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199610)9:7<305::aid-nbm434>3.0.co;2-d
Subject(s) - oxygenation , chemistry , nuclear magnetic resonance , magnetic resonance imaging , muscle tissue , proton magnetic resonance , phosphocreatine , in vivo , phosphorus , oxygen , human muscle , analytical chemistry (journal) , anatomy , medicine , skeletal muscle , chromatography , biology , energy metabolism , physics , microbiology and biotechnology , organic chemistry , radiology
A pressure cuff was applied to the legs of two human volunteers in order to stop any blood supply for a period of about 30 min. The affected muscle was monitored using proton magnetic resonance imaging (MRI), phosphorus magnetic resonance spectrometry (MRS) and near infrared (NIR) specroscopy before, during and after this procedure. The internal temperature of the tissue was also measured. The phase of water protons in muscle showed changes that were not accounted for by the measured temperature, but which correlated with the large increase in deoxyhaemoglobin and deoxymyoglobin observed with NIR as well as the decrease in PCr and increase in P i observed with MRS. Little or no change was found in proton density or T 2 * . These results show that in vivo measurements of temperature using the chemical shift method may be confounded by changes in tissue oxygenation. They also show that T 2 * is an insensitive measure of changes in tissue oxygenation. © 1996 John Wiley & Sons, Ltd.