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The Protective Effect of MK‐801 on Infarct Development over a Period of 24 h as Assessed by Diffusion‐Weighted Magnetic Resonance Imaging
Author(s) -
Gill R.,
Sibson N. R.,
Maskell L.,
Carpenter T. A.,
Hall L. D.,
Pickard J. D.
Publication year - 1996
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199609)9:6<241::aid-nbm432>3.0.co;2-7
Subject(s) - period (music) , magnetic resonance imaging , nuclear magnetic resonance , diffusion mri , diffusion , diffusion weighted magnetic resonance imaging , medicine , physics , radiology , thermodynamics , acoustics
Diffusion‐weighted MRI has been used to investigate therapeutic intervention with MK‐801 in an animal model of permanent focal cerebral ischaemia. The animals were imaged continuously for 4 h and again at 24 h following occlusion of the middle cerebral artery (MCA) allowing the development of the ischaemic lesion to be monitored continuously in the same animals. An increased DWI signal, seen as a region of hyperintensity, was detected 1 h after MCA‐occlusion in the lateral cortex and caudate nucleus in both control and MK‐801 (administered at a dose of 3 mg/kg i.p. 5 min post‐ischaemia) treated animals. However, the volume of hemispheric and cortical hyperintensity was smaller in the MK‐801‐treated animals. The area of hyperintensity progressively increased in the control group over the 4 h imaging time and there was also an increase in the area of hyperintensity between 4 and 24 h. At these time points the area of hyperintensity encompassed the dorsolateral cortex and caudate nucleus. MK‐801 treated animals also demonstrated some progressive increase in the area of hyperintensity between 1 and 3 h, but no significant increase in the area of hyperintensity was seen after this time. The hyperintense regions at 4 and 24 h were restricted to the so‐called "core areas' of the lesion in MK‐801‐treated animals. Thus, using DWI the tissue "at risk' following ischaemia could be identified and the protective effect of therapeutic intervention demonstrated