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Relationships between Fiber Composition and NMR Measurements in Human Skeletal Muscle
Author(s) -
Takahashi Hideyuki,
Kuno Shinya,
Katsuta Shigeru,
Shimojo Hitoshi,
Masuda Kazumi,
Yoshioka Hiroshi,
Anno Izumi,
Itai Yuji
Publication year - 1996
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199602)9:1<8::aid-nbm387>3.0.co;2-f
Subject(s) - phosphocreatine , chemistry , skeletal muscle , myosin atpase , pi , fiber , myosin , nuclear magnetic resonance spectroscopy , vastus lateralis muscle , fiber type , composition (language) , nuclear magnetic resonance , adenosine triphosphate , atpase , biochemistry , anatomy , biology , endocrinology , enzyme , energy metabolism , linguistics , philosophy , physics , organic chemistry
The purpose of this study was to examine the relationships between the relative contents of phosphocreatine (PCr), inorganic phosphate (Pi), β‐adenosine triphosphate (ATP), and transverse relaxation time ( T 2 ) with fiber composition, which determined histochemically in the human skeletal muscle. The vastus lateralis muscles of 28 volunteers were subjected to phosphorus nuclear magnetic resonance ( 31 P NMR) spectroscopy, magnetic resonance imaging (MRI) and muscle biopsy. Muscle fibers were divided into type I and type II fibers using myosin ATPase stain. A wide range of fiber composition levels were observed in the subjects (27.3–74.6% type I fibers). The PCr/ATP, Pi/ATP and (PCr+Pi)/ATP ratios were positively related to the percentage of type II fibers ( r =0.695, p <0.001, r =0.429, p <0.05 and r =0.773, p <0.001, respectively). There was no correlation between fiber composition and the PCr/Pi ratio ( r 0.127, n.s.) or intracellular pH ( r =0.305, n.s.). Moreover, no correlation was found between T 2 and fiber type ( r =0.144, n.s.). These results suggest that 31 P NMR can detect the differences in relative content of phosphates between type I and type II fibers, thereby noninvasively evaluating fiber composition in human skeletal muscle.