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Change in Chemical Shift and Splitting of 31 P γ‐ATP Signal in Human Skeletal Muscle During Exercise and Recovery
Author(s) -
Widmaier Stefan,
Jung WulfIngo,
Bunse Michael,
van Erckelens Franz,
Dietze Günther,
Lutz Otto
Publication year - 1996
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/(sici)1099-1492(199602)9:1<1::aid-nbm384>3.0.co;2-8
Subject(s) - biceps , chemical shift , signal (programming language) , pi , chemistry , skeletal muscle , biophysics , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , anatomy , biochemistry , biology , physics , stereochemistry , computer science , programming language
Proton decoupled 31 P in vivo NMR spectroscopy of the human finger flexor muscles was performed during exercise and recovery using a 1.5 T whole‐body imager. Predominantly the γ‐ATP signal shows a splitting caused by different signal contributions with chemical shifts that vary independently. Studies on the human gastrocnemius and biceps femoris muscle were undertaken to investigate the appearance of the splitting in these muscles as well. In all cases more than one signal contribution was found which might represent the different muscle fibre types and their recruitment pattern following exercise. An analysis of the chemical shifts of ATP results in changes of up to 0.4 ppm and 0.1 ppm for δγ‐ and δβ‐ATP, respectively. Based solely on the chemical shifts of the ATP 31 P signals the tissue pH value following exercise was determined. The result was in good agreement with the value derived from δP i .