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7 Li and 23 Na NMR studies of transmembrane cation transport mediated by ionophore lasalocid A
Author(s) -
Juvvadi Padmaja,
Kalapaty Easwaran
Publication year - 1998
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/(sici)1099-1387(199802)4:1<15::aid-psc96>3.0.co;2-e
Subject(s) - lasalocid , ionophore , chemistry , lithium (medication) , aqueous solution , reagent , inorganic chemistry , ion transporter , vesicle , ion , membrane , organic chemistry , biochemistry , medicine , endocrinology
Ion transport across phospholipid vesicles was studied by 7 Li and 23 Na‐NMR using an aqueous anionic paramagnetic shift reagent, dysprosium nitrilotriacetate [Dy(NTA) 2 ] 3− , mediated by ionophores, lasalocid A and A23187. The intra‐ and extracellular 7 Li and 23 Na‐NMR signals were well separated (20 Hz) at m M concentration of the shift reagent. The observed data on the rate constant for lithium transport across DPPC vesicles at various concentrations of the ionophores indicated that lasalocid A is a more efficient carrier for lithium ion compared with the sodium ion transport by this ionophore, while A23187 was not specific to either of the ions (Li or Na). ©1998 European Peptide Society and John Wiley & Sons, Ltd.