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Fluorescence correlation spectroscopy: molecular recognition at the single molecule level
Author(s) -
Van Craenenbroeck E.,
Engelborghs Y.
Publication year - 2000
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(200003/04)13:2<93::aid-jmr492>3.0.co;2-6
Subject(s) - fluorescence correlation spectroscopy , fluorescence , fluorescence cross correlation spectroscopy , fluorescence spectroscopy , fluorescence microscope , single molecule experiment , biophysics , chemistry , molecule , spectroscopy , molecular recognition , microscopy , in vivo , biology , optics , physics , organic chemistry , genetics , quantum mechanics
Fluorescence correlation spectroscopy (FCS) is a fluorescence microscopy technique that allows the study of molecular interactions in extremely low volumes, at nanomolar concentrations, even when binding is not accompanied by a fluorescence change. It can be applied directly in living cells. FCS clearly considerably extends the possibilities of the classical techniques used in molecular recognition studies and can be considered to belong to a growing group of techniques that allow detection at the single molecule level. In this review, several applications of FCS, both in vitro and in vivo , will be discussed. Copyright © 2000 John Wiley & Sons, Ltd.