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Thermodynamic analysis of protein interactions with biosensor technology
Author(s) -
Roos Håkan,
Karlsson Robert,
Nilshans Helena,
Persson Anette
Publication year - 1998
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199812)11:1/6<204::aid-jmr424>3.0.co;2-t
Subject(s) - isothermal microcalorimetry , enthalpy , thermodynamics , chemistry , biosensor , entropy (arrow of time) , reaction rate constant , kinetics , biochemistry , physics , quantum mechanics
A methodology using biosensor technology for combined kinetic and thermodynamic analysis of biomolecular interactions is described. Rate and affinity constants are determined with BIAcore. Thermodynamics parameters, changes in free energy, enthalpy and entropy, are evaluated from equilibrium data and by using rate constants and transition state theory. The methodology using van't Hoff theory gives complementary information to microcalorimetry, since only the direct binding is measured with BIAcore whereas microcalorimetry measures all components, including e.g. hydration effects. Furthermore, BIAcore gives possibilities to gain new information by thermodynamic analysis of the rate constants. Copyright © 1998 John Wiley & Sons, Ltd.