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Electrochemical immunosensor for the detection of atrazine
Author(s) -
López Miguel A.,
Ortega Fidel,
Domínguez Elena,
Katakis Ioanis
Publication year - 1998
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199812)11:1/6<178::aid-jmr417>3.0.co;2-5
Subject(s) - horseradish peroxidase , amperometry , redox , atrazine , electrochemistry , electrode , chemistry , biosensor , peroxidase , chromatography , combinatorial chemistry , inorganic chemistry , enzyme , organic chemistry , biochemistry , pesticide , biology , agronomy
An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy) 2 Cl was co‐immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron‐conducting hydrogel. In a competitive assay the occurrence of the antibody–antigen reaction on the surface of the sensing film was detected through the ‘electrical wiring’ of the redox centres of antigen‐labelled horseradish peroxidase and the electrode surface in the presence of H 2 O 2 at 0.1 V (vsAg/AgCl). Copyright © 1998 John Wiley & Sons, Ltd.