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Measurement of antigen–antibody interactions with biosensors
Author(s) -
Regenmortel Marc H. V. Van,
Altschuh Danièle,
Chatellier Jean,
Christensen Lars,
RaufferBruyère Nathalie,
RichaletSecordel Pascale,
Witz Jean,
ZederLutz Gabrielle
Publication year - 1998
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199812)11:1/6<163::aid-jmr414>3.0.co;2-u
Subject(s) - biosensor , surface plasmon resonance , epitope , antigen , chemistry , refractive index , nanotechnology , antibody , biophysics , biochemistry , materials science , biology , optoelectronics , immunology , nanoparticle
The introduction in 1990 of a new biosensor technology based on surface plasmon resonance has revolutionized the measurement of antigen–antibody binding interactions. In this technique, one of the interacting partners is immobilized on a sensor chip and the binding of the other is followed by the increase in refractive index caused by the mass of bound species. The following immunochemical applications of this new technology will be described: (1) functional mapping of epitopes and paratopes by mutagenesis; (2) analysis of the thermodynamic parameters of the interaction; (3) measurement of the concentration of biogically active molecules; (4) selection of diagnostic probes. Copyright © 1998 John Wiley & Sons, Ltd.