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Capillary affinophoresis as a versatile tool for the study of biomolecular interactions: a mini‐review
Author(s) -
Shimura Kiyohito,
Kasai Kenichi
Publication year - 1998
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199812)11:1/6<134::aid-jmr409>3.0.co;2-g
Subject(s) - capillary electrophoresis , biomolecule , affinity electrophoresis , chemistry , electrophoresis , ligand (biochemistry) , molecule , nanotechnology , stoichiometry , combinatorial chemistry , chromatography , materials science , biochemistry , organic chemistry , affinity chromatography , receptor , enzyme
Combination of capillary electrophoresis and bioaffinity interaction gave rise to powerful research tools for analyzing molecular recognition. They take advantages of the electrophoretic behavior of the complex formed between a target biomolecule and a specially designed mobile ligand molecule (affinophore or affinity probe), and enable detection of complex formation, determination of the equilibrium constants and stoichiometry, etc. Copyright © 1998 John Wiley & Sons, Ltd.