Premium
DNA binding properties of minor groove binders and their influence on the topoisomerase II cleavage reaction
Author(s) -
Bell Achim,
Kittler Leonhard,
Löber Günter,
Zimmer Christoph
Publication year - 1997
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199711/12)10:6<245::aid-jmr367>3.0.co;2-3
Subject(s) - netropsin , topoisomerase , dna , cleavage (geology) , minor groove , chemistry , ligand (biochemistry) , binding site , titration , groove (engineering) , stereochemistry , biophysics , microbiology and biotechnology , biochemistry , biology , receptor , paleontology , inorganic chemistry , materials science , fracture (geology) , metallurgy
We present titrations of the human δβ‐globin gene region with DNA minor groove binders netropsin, bisnetropsin, distamycin, chromomycin and four bis‐quaternary ammonium compounds in the presence of calf thymus topoisomerase II and DNase I. With increasing ligand concentration, stimulation and inhibition of enzyme activity were detected and quantitatively evaluated. Additionally we show a second type of stimulation, the appearance of strong new topoisomerase II cleavage sites at high ligand concentrations. The specific binding sites of the minor groove binders of the DNA sequence and their microscopic binding constants were determined from DNase I footprints. A binding mechanism for minor groove binders is proposed in order to explain these results especially when ligand concentration is increased. © 1998 John Wiley & Sons, Ltd.