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Diazotizable supports of potential interest as affinity chromatography matrices
Author(s) -
Beneš M. J.,
Lenfeld J.,
Přádová O.
Publication year - 1996
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199634/12)9:5/6<701::aid-jmr325>3.0.co;2-d
Subject(s) - chemistry , reactivity (psychology) , amine gas treating , azo coupling , reagent , imidazole , epoxide , functional group , combinatorial chemistry , ligand (biochemistry) , coupling reaction , matrix (chemical analysis) , surface modification , organic chemistry , catalysis , chromatography , polymer , medicine , receptor , pathology , biochemistry , alternative medicine
Immobilization of affinity ligands, proteins, enzymes and other functional groups by azo coupling is based on the high reactivity of the support‐carrying diazonium groups towards both low‐ and high‐molecular weight compounds containing certain groupings such as phenols, imidazole and some other heterocycles, thiols and amines. The precursor of diazonium group is a diazotizable amine on the matrix. Remarkable progress in its preparation was achieved by application of (4‐amino‐phenyl)‐(2‐sulphatoxyethyl) sulphone‐type reagents for functionalization of the matrix. A similar type of amine precursor is prepared by the reaction of monosubstituted sulphanilamide with epoxide‐containing matrix. The presence of a SO 2 group in the para position to diazonium group of these supports (after diazotization) enhances reactivity in azo coupling. ‘Reversed’ azo coupling is the reaction of a matrix containing functional groups capable of reacting with diazonium groups of a ligand. Preparation of a suitable matrix and examples of diazotizable ligands are given.