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Some recent developments in the preparation of novel recognition systems: A recognition site for the selective catalysis of an aldol condensation using molecular imprinting and specific affinity motifs for α‐chymotrypsin using a phage display peptide library
Author(s) -
Nicholls Ian A.,
Matsui Jun,
Krook Margareta,
Mosbach Klaus
Publication year - 1996
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199634/12)9:5/6<652::aid-jmr316>3.0.co;2-6
Subject(s) - molecular imprinting , molecular recognition , chemistry , chymotrypsin , combinatorial chemistry , aldolase a , aldol condensation , imprinting (psychology) , acetaldehyde , phage display , peptide , biochemistry , catalysis , enzyme , organic chemistry , selectivity , molecule , trypsin , gene , ethanol
Molecular imprinting and phage display library technologies are rapidly being accepted as useful techniques for the generation of ligand‐selective recognition motifs. The use of molecular imprinting to produce a novel type II aldolase mimic selective for the cobalt(II)‐mediated aldol condensation of benzophenone and acetaldehyde is reported here. Furthermore, peptide motifs have been identified which are acting as 'affinity ligands' selective for the recognition of the enzyme α‐chymotrypsin using phage display techniques.