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Polymer‐shielded dye‐affinity chromatography
Author(s) -
Mattiasson Bo,
Galaev Igor Yu.,
Garg Nandita
Publication year - 1996
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199634/12)9:5/6<509::aid-jmr293>3.0.co;2-y
Subject(s) - polymer , vinyl alcohol , electromagnetic shielding , shielded cable , affinity chromatography , matrix (chemical analysis) , chemistry , chromatography , thermoresponsive polymers in chromatography , column chromatography , materials science , chromatography column , organic chemistry , computer science , composite material , telecommunications , enzyme
Polymer‐shielded dye‐affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water‐soluble polymer such as poly(vinylpyrrolidone) or poly(vinyl alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.