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Immobilization of streptavidin for immunosensors on nanostructured surfaces
Author(s) -
Kossek S.,
Padeste C.,
Tiefenauer L.
Publication year - 1996
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199634/12)9:5/6<485::aid-jmr288>3.0.co;2-r
Subject(s) - streptavidin , ferrocene , biotinylation , analyte , nanotechnology , biosensor , chemistry , silicon , modular design , electrode , amperometry , combinatorial chemistry , electrochemistry , materials science , biotin , chromatography , computer science , biochemistry , organic chemistry , operating system
A modular immunosensor device based on silicon technology with electrochemical detection is presented. The sensor surface is functionalized with the antigen estradiol using the streptavidin–biotin system. The immobilized antigen is recognized by ferrocene‐labeled antibody molecules in a competitive assay format. The ferrocene redox centers can be detected electrochemically. In order to facilitate the electron transfer, a gold layer structured in nanometer dimensions is used as an electrode. The functionalized silicon chip will be integrated into a micromachined fluid transport system. The resulting immunosensor device for estradiol represents a prototype allowing easy adaptation to other biotinylated analytes since the immobilized streptavidin serves as a universal anchor.