Premium
Preliminary investigations of an amperometric oligosaccharide dehydrogenase‐based electrode for the detection of glucose and some other low molecular weight saccharides
Author(s) -
Ruzgas T.,
Csöregi E.,
Katakis I.,
Kenausis G.,
Gorton L.
Publication year - 1996
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199634/12)9:5/6<480::aid-jmr287>3.0.co;2-8
Subject(s) - chemistry , cellobiose , maltotriose , cellobiose dehydrogenase , maltose , amperometry , chromatography , ethylene glycol , oligosaccharide , xylose , fructose , biochemistry , galactose , monosaccharide , lactose , biosensor , enzyme , organic chemistry , electrode , fermentation , electrochemistry , cellulase
Biosensors for the determination of sugars were constructed using oligosaccharide dehydrogenase (ODH) and soluble phenazine methosulfate (PMS) or an osmium‐based three‐dimensional redox hydrogel. In the latter case the enzyme and poly(1‐vinylimidazole) complexed with osmium (4,4′‐dimethylbpy) 2 Cl were cross‐linked with poly(ethylene glycol) diglycidyl ether. Both electrode configurations showed similar sensitivities for glucose in the range between 8 and 21 μAm M −1 cm −2 . The responses for 10 mono and oligosaccharides were studied. There was no response for fructose. In the concentration range 0.1–20 m M the relative sensitivities were determined for arabinose (96%), xylose (3%), mannose (50%), galactose (11%), glucose (100%), maltose (24%), lactose (12%), cellobiose (34%) and maltotriose (10%).