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Salicylaldehyde–metal–amino acid ternary complex: A new tool for immobilized metal affinity chromatography
Author(s) -
Leibler D.,
Rabinkov A.,
Wilchek M.
Publication year - 1996
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199634/12)9:5/6<375::aid-jmr268>3.0.co;2-x
Subject(s) - salicylaldehyde , chemistry , concanavalin a , imidazole , chromatography , affinity chromatography , metal , elution , copper , nuclear chemistry , polymer chemistry , organic chemistry , biochemistry , schiff base , in vitro , enzyme
An immobilized salicylaldehyde (sal) was used to build various salicylaldehyde–copper–amino acid (Sal–Cu–AA) complexes which are stable at a range of pH values (2.0–11.0). The complexes were found to bind protein molecules as IMAC resins. Thirteen proteins were examined for their binding to a Sal–Cu–Gly column. The efficacy of the Sal–Cu–AA resin for protein separation were demonstrated by two examples. The first was a new purification process for garlic lectins from garlic crude extract. It seems that in this case the Sal–Cu–AA resins were more selective than IDA resin. The second was immobilization of concanavalin A (Con A) on the resin and using the immobilzed Con A for affinity chromatography of mannose‐rich glycoprotein ovalbumin. The Con A could be later eluted with EDTA or imidazole and the Sal‐containing polymer could be recharged again for further use.