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Sequence/structure selective thermal and photochemical cleavage of yeast‐tRNA Phe by UO 2 2+
Author(s) -
Nielsen Peter E.,
Møllegaard Niels Erik
Publication year - 1996
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/(sici)1099-1352(199605)9:3<228::aid-jmr243>3.0.co;2-r
Subject(s) - uranyl , chemistry , cleavage (geology) , yeast , metal , transfer rna , stereochemistry , binding site , crystallography , divalent , rna , metal ions in aqueous solution , stoichiometry , molecule , ion , biochemistry , biology , organic chemistry , paleontology , fracture (geology) , gene
The uranyl(VI) ion, UO 2 2+ , cleaves yeast tRNA Phe both thermally and photochemically. Photochemical cleavage takes place at all positions but exhibits maxima at G 10 , G 18 , G 30 , A 38 , C 49 and A 62 . Furthermore, in the presence of stoichiometric concentrations of citrate, the cleavage is generally suppressed except that strong cleavage at positions G 10 and C 48 –U 50 persists, indicating the presence of a high‐affinity metal‐ion binding site. It is proposed that these photocleavage sites reflect the tertiary structure of the yeast tRNA Phe molecule in terms of D‐loop/T‐loop interaction and anticodon loop conformation and that uranyl‐mediated photocleavage of RNA may be used as a probe of RNA tertiary structure, and in particular for identifying binding sites for divalent metal ions. Thus a high‐affinity metal‐ion binding site is inferred in the Rcentral pocket' formed by the D‐loop, the T‐loop and the acceptor stem.