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A fast and convenient method for robotic preparation of [ 11 C]flumazenil avoiding HPLC purification
Author(s) -
Krasikova Raisa,
Fedorova Olga,
Korsakov Michail,
Någren Kjell,
Maziere Bernard,
Halldin Christer
Publication year - 2000
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(200005)43:6<613::aid-jlcr346>3.0.co;2-u
Subject(s) - chemistry , flumazenil , high performance liquid chromatography , chromatography , cartridge , alum , organic chemistry , antagonist , mechanical engineering , biochemistry , receptor , engineering
A new approach for robotic preparation of [ 11 C]flumazenil with recovery of the final product by SPE technique has been developed. A hydrogen‐bond‐assisted methylation in a suspension of potassium fluoride absorbed on alumina was followed by purification using a standard Sep‐Pak Neutral Alum N Light cartridge. As a result, the contamination of the final product with its precursor Ro 15‐5528 was reduced to similar levels as obtained by traditional semi‐preparative HPLC. The synthesis of [ 11 C]flumazenil was operated by the commercially available robot Anatech RB‐86. The formulated solution of [ 11 C]flumazenil was obtained within 18 min starting from trapping of [ 11 C]methyl iodide. The simplicity of this method and the use of disposable materials for purification instead of HPLC may be advantageous for routine clinical use. Copyright © 2000 John Wiley & Sons, Ltd.