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[ 11 C]Methylation on a C 18 Sep‐Pak cartridge: a convenient way to produce [ N ‐methyl‐ 11 C]choline
Author(s) -
Pascali Claudio,
Bogni Anna,
Itawa Ren,
Cambiè Mara,
Bombardieri Emilio
Publication year - 2000
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(200002)43:2<195::aid-jlcr316>3.0.co;2-p
Subject(s) - chemistry , cartridge , choline , elution , yield (engineering) , methylation , chromatography , vial , ion exchange resin , nuclear chemistry , radiochemistry , organic chemistry , biochemistry , mechanical engineering , materials science , engineering , metallurgy , gene
[ N ‐Methyl‐ 11 C]choline has been simply and efficiently labelled at room temperature by solid supported [ 11 C]methylation of 2‐dimethylaminoethanol on a commercial C 18 Sep‐Pak Light cartridge. The labelled product was retained on a cation exchange resin (Sep‐Pak Plus Accell Plus CM) and washed with ethanol and water to remove any excess precursor. Then [ N ‐methyl‐ 11 C]choline was desorbed from the cartridge by elution with saline, passed through a 0·2 μm sterile filter and collected in a sterile vial. Overall, the total synthesis time was 12 min from the end of bombardment (EOB). The radiochemical yield from [ 11 C]CO 2 , decay corrected (EOB) was 87% and the radiochemical purity was greater than 99%. Very conveniently, the automated apparatus employed is a very simple modification of the system routinely used for the synthesis of L‐[ S ‐methyl‐ 11 C]methionine. Copyright © 2000 John Wiley & Sons, Ltd.