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High efficiency preparation of L‐[ S ‐methyl‐ 11 C]methionine by on‐column [ 11 C]methylation on C18 Sep‐Pak
Author(s) -
Pascali Claudio,
Bogni Anna,
Iwata Ren,
Decise Donatella,
Crippa Flavio,
Bombardieri Emilio
Publication year - 1999
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(199908)42:8<715::aid-jlcr224>3.0.co;2-3
Subject(s) - chemistry , methylation , methionine , column (typography) , chromatography , biochemistry , amino acid , dna , structural engineering , connection (principal bundle) , engineering
A novel approach to the synthesis of L‐[ S ‐methyl‐ 11 C]methionine ([ 11 C]MET) is described which involves a commercial C18 Sep‐Pak Plus as a solid‐phase support material for the [ 11 C]methylation step. The present method, which uses [ 11 C]CH 3 I produced by the classical LiA1H 4 /HI route, supplies [ 11 C]MET ready for injection within 11 min from the end of bombardment (EOB) with a radiochemical yield, decay corrected at EOB, of 78% and a radiochemical purity at the end of synthesis (EOS) higher than 99%. The required setup is extremely simple and easy to automate and can be reset for a further synthesis within a few minutes. Moreover, due to its versatility, the method can be utilized for the production of other radiopharmaceuticals requiring a simple [ 11 C]methylation. Copyright © 1999 John Wiley & Sons, Ltd.