Premium
The enzymatic synthesis of isotopically labelled penicillin Ns with isopenicillin N synthase
Author(s) -
Baldwin Jack E.,
Adlington Robert M.,
Crouch Nicholas P.,
Pereira Inês A. C.
Publication year - 1998
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(199812)41:12<1145::aid-jlcr159>3.0.co;2-2
Subject(s) - tripeptide , chemistry , penicillin , enzyme , biosynthesis , stereochemistry , chemical synthesis , biochemistry , combinatorial chemistry , antibiotics , amino acid , in vitro
The preparation of isotopically labelled penicillin Ns using a chemico‐enzymatic approach is described. This route involves the chemical synthesis of variously labelled D,L,D ‐aminoadipoyl‐cysteinyl‐valine tripeptides via well established facile protocols and concludes with the conversion of these tripeptides directly into penicillin Ns by the action of recombinant isopenicillin N synthase. Milligram quantities of isotopically labelled penicillin Ns, which would otherwise represent very challenging and expensive synthetic targets, are readily accessible from this route. Copyright © 1998 John Wiley & Sons, Ltd.