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A qualitative method for assessing the 14 C labelling position in [ 14 C]‐dimethyl aminoantipyrine (aminopyrine)
Author(s) -
Levêque Philippe,
Gallez Bernard
Publication year - 1998
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(1998100)41:10<959::aid-jlcr149>3.0.co;2-9
Subject(s) - labelling , chemistry , demethylation , formaldehyde , horseradish peroxidase , peroxidase , chromatography , biochemistry , enzyme , gene expression , dna methylation , gene
[ 14 C]‐Aminopyrine is a well known compound which is widely used for the evaluation of the hepatic function (breath test). As this breath test is based upon N‐demethylation, it is essential to verify the labelling position in order to guarantee relevant results. We propose an enzymatic method based on collection of 14 CO 2 produced after specific in vitro N‐demethylation in a medium containing horseradish peroxidase, formaldehyde dehydrogenase and formate dehydrogenase. This assay allows for a qualitative monitoring of the labelling position.Copyright © 1998 John Wiley & Sons, Ltd.