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Synthesis of [3α‐ 3 H]‐dehydroepiandrosterone and [3α‐ 3 H]‐pregnenolone
Author(s) -
Tait A. D.
Publication year - 1998
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(199803)41:3<221::aid-jlcr70>3.0.co;2-y
Subject(s) - pregnenolone , chemistry , dehydroepiandrosterone , sodium borohydride , tritium , steroid , enzyme , borohydride , stereochemistry , endocrinology , biochemistry , androgen , hormone , catalysis , medicine , physics , nuclear physics
[3α‐ 3 H]‐Dehydroepiandrosterone ([3α‐ 3 H]‐3β‐hydroxy‐5‐androsten‐17‐one) and [3α‐ 3 H]‐pregnenolone ([3α‐ 3 H]‐3β‐hydroxy‐5‐pregnen‐20‐one) were prepared by selective reduction of 3‐keto‐5‐ene intermediates with tritiated sodium borohydride. These were used as substrates to set up a tritium release assay for 3β‐hydroxysteroid oxido‐reductase and 5→4‐ene isomerase (3β‐HSD) which is a key enzyme in steroidogenesis. © 1998 John Wiley & Sons, Ltd.