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Direct 99m Tc labeling of monoclonal antibodies: evaluation of reducing agents and HPLC analysis
Author(s) -
Liu Ning,
Jin Jiannan,
Mo Shangwu,
Chen Hengliu,
Yu Yanping,
Xu Bingxun,
Chen Qi
Publication year - 1998
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(199801)41:1<37::aid-jlcr51>3.0.co;2-g
Subject(s) - chemistry , dithiothreitol , monoclonal antibody , high performance liquid chromatography , chromatography , yield (engineering) , antibody , reducing agent , column chromatography , biochemistry , organic chemistry , enzyme , materials science , metallurgy , immunology , biology
Human IgG was used as a model antibody and the results for direct 99m Tc labeling of IgG using 2‐mercaptoethanol (2‐ME), dithiothreitol (DTT) and ascorbin acid (AA) as reducing agents were evaluated. DTT was chosen as reducing agent and an intact monoclonal antibody 3H11 was labeled with 99m Tc with a labeling yield of more than 98% while its Fab fragment was labeled with 99m Tc in less than 35% yield. The 99m Tc labeled antibodies were analysed by HPLC on a size exclusion column with an eluent of 0.1 mol/L PBS(pH 7.0), and the in vitro stability of 99m Tc‐3H11 was investigated. HPLC analysis revealed that 99m Tc‐3H11 Fab is not in a singular structure, and the procedure for direct 99m Tc labeling of antibody's Fab Fragment should be further investigated. © 1998 John Wiley & Sons, Ltd.