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A biosynthetic route to [ 14 C]‐labelled rhamnolipids
Author(s) -
Schenk Torsten,
Breitschwerdt Andrea,
Kessels Gerhild,
Schuphan Ingolf,
Schimdt Burkhard
Publication year - 1997
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(199708)39:8<705::aid-jlcr11>3.0.co;2-7
Subject(s) - chemistry , chromatography , fraction (chemistry) , rhamnose , high performance liquid chromatography , extraction (chemistry) , biochemistry , polysaccharide
After feeding 200 mg of [U‐ 14 C]‐ D ‐glucose (8.25 MBq/mmol; 9.16 Mbq) to a culture of Pseudomonas aeruginosa DSM 2659, the rhamnolipids RL‐1 and RL‐2 produced by the microorganism were obtained in uniformely 14 C‐labelled form as crude fraction by extraction of the medium. The fraction was analyzed by TLC and HPLC. Cleavage with HCl gave rhamnose (identified by TLC) and β‐hydroxydecanoic acid (identified by TLC and GC‐MS). The radiochemical yields were 1.19% for RL‐1 and 1.15% for RL‐2; specific activities amounted to 23.191 and 27.062 MBq/mmol, respectively. © John Wiley & Sons, Ltd.