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New chemo‐enzymic synthesis of very high specific radioactivity [ 35 S] (S) methionine [1].
Author(s) -
Boullais Claude,
Riva Michel,
Noel JeanPierre
Publication year - 1997
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(199707)39:7<621::aid-jlcr13>3.0.co;2-i
Subject(s) - chemistry , methionine , yield (engineering) , homoserine , high performance liquid chromatography , specific activity , thiol , chromatography , organic chemistry , biochemistry , amino acid , enzyme , materials science , quorum sensing , virulence , metallurgy , gene
[ 35 S] H 2 SO 4 was quantitatively reduced to [ 35 S] H 2 S with a pretreated mixture of Hl/HCl/H 3 PO 2 . [ 35 S] H 2 S was used in a (S) homoserine sulfhydrylase [EC.4.2.99.10] catalysed thiol exchange reaction with O‐acetyl‐(S) homoserine to afford [ 35 S] (S) homocysteine. The latter was methylated (CH 3 I/NaOH) in situ giving rise to [ 35 S] (S) methionine which was purified by HPLC. Overall yield 10‐15%; specific radioactivity : > 46 TBq/mmol e.g. > 1243 Ci/mmol; chemical and radiochemical purities > 98%. © 1997 John Wiley & Sons, Ltd.