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Synthesis of high specific activity tritium labelled [2‐ 3 H]‐adenosine‐5′‐triphosphate
Author(s) -
Jaiswal Devendra K.,
Morimoto Hiromi,
Trump Eric L.,
Williams Philip G.,
Wemmer David E.
Publication year - 1996
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/(sici)1099-1344(199608)38:8<743::aid-jlcr884>3.0.co;2-f
Subject(s) - chemistry , tritium , adenosine triphosphate , labelling , guanosine , specific activity , yield (engineering) , radiochemistry , chemical synthesis , guanosine triphosphate , phosphate , nuclear chemistry , biochemistry , enzyme , in vitro , gtp' , materials science , metallurgy , physics , nuclear physics
A procedure for high level tritium labelling at the C2‐H position of adenosine 5′‐triphosphate ([2‐ 3 H]‐ATP, 1 ), based on the tritiodehalogenation reaction of 2‐bromoadenosine 5′‐triphosphate ( 2 ) has been elaborated. This precursor was prepared in a six‐step synthesis from guanosine. The tritiodehalogenation of ( 2 ) for three hours over palladium oxide in phosphate buffer yielded tritium labelled ATP with high specific activity, in good chemical yield.